Interaction between the moss Physcomitrella patens and Phytophthora: a novel pathosystem for live-cell imaging of subcellular defence.
Identifieur interne : 000B96 ( Main/Exploration ); précédent : 000B95; suivant : 000B97Interaction between the moss Physcomitrella patens and Phytophthora: a novel pathosystem for live-cell imaging of subcellular defence.
Auteurs : Elysa J R. Overdijk [Pays-Bas] ; Jeroen De Keijzer [Pays-Bas] ; Deborah De Groot [Pays-Bas] ; Charikleia Schoina [Pays-Bas] ; Klaas Bouwmeester [Pays-Bas] ; Tijs Ketelaar [Pays-Bas] ; Francine Govers [Pays-Bas]Source :
- Journal of microscopy [ 1365-2818 ] ; 2016.
Descripteurs français
- KwdFr :
- Actines (métabolisme), Bryopsida (cytologie), Bryopsida (parasitologie), Cytoplasme (métabolisme), Espace intracellulaire (MeSH), Maladies des plantes (parasitologie), Microscopie (méthodes), Noyau de la cellule (métabolisme), Paroi cellulaire (métabolisme), Phytophthora (pathogénicité), Phytophthora (physiologie), Survie cellulaire (MeSH).
- MESH :
- cytologie : Bryopsida.
- métabolisme : Actines, Cytoplasme, Noyau de la cellule, Paroi cellulaire.
- méthodes : Microscopie.
- parasitologie : Bryopsida, Maladies des plantes.
- pathogénicité : Phytophthora.
- physiologie : Phytophthora.
- Espace intracellulaire, Survie cellulaire.
English descriptors
- KwdEn :
- Actins (metabolism), Bryopsida (cytology), Bryopsida (parasitology), Cell Nucleus (metabolism), Cell Survival (MeSH), Cell Wall (metabolism), Cytoplasm (metabolism), Intracellular Space (MeSH), Microscopy (methods), Phytophthora (pathogenicity), Phytophthora (physiology), Plant Diseases (parasitology).
- MESH :
- chemical , metabolism : Actins.
- cytology : Bryopsida.
- metabolism : Cell Nucleus, Cell Wall, Cytoplasm.
- methods : Microscopy.
- parasitology : Bryopsida, Plant Diseases.
- pathogenicity : Phytophthora.
- physiology : Phytophthora.
- Cell Survival, Intracellular Space.
Abstract
Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.
DOI: 10.1111/jmi.12395
PubMed: 27027911
Affiliations:
- Pays-Bas
- Gueldre (province), Utrecht (province)
- Utrecht, Wageningue
- Université d'Utrecht, Université de Wageningue
Links toward previous steps (curation, corpus...)
Le document en format XML
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patens and Phytophthora: a novel pathosystem for live-cell imaging of subcellular defence.</title><author><name sortKey="Overdijk, Elysa J R" sort="Overdijk, Elysa J R" uniqKey="Overdijk E" first="Elysa J R" last="Overdijk">Elysa J R. Overdijk</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Cell Biology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="De Keijzer, Jeroen" sort="De Keijzer, Jeroen" uniqKey="De Keijzer J" first="Jeroen" last="De Keijzer">Jeroen De Keijzer</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Cell Biology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="De Groot, Deborah" sort="De Groot, Deborah" uniqKey="De Groot D" first="Deborah" last="De Groot">Deborah De Groot</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Schoina, Charikleia" sort="Schoina, Charikleia" uniqKey="Schoina C" first="Charikleia" last="Schoina">Charikleia Schoina</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Plant-Microbe Interactions, Utrecht University, Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Plant-Microbe Interactions, Utrecht University, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Ketelaar, Tijs" sort="Ketelaar, Tijs" uniqKey="Ketelaar T" first="Tijs" last="Ketelaar">Tijs Ketelaar</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Cell Biology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author></analytic><series><title level="j">Journal of microscopy</title><idno type="eISSN">1365-2818</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actins (metabolism)</term><term>Bryopsida (cytology)</term><term>Bryopsida (parasitology)</term><term>Cell Nucleus (metabolism)</term><term>Cell Survival (MeSH)</term><term>Cell Wall (metabolism)</term><term>Cytoplasm (metabolism)</term><term>Intracellular Space (MeSH)</term><term>Microscopy (methods)</term><term>Phytophthora (pathogenicity)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Actines (métabolisme)</term><term>Bryopsida (cytologie)</term><term>Bryopsida (parasitologie)</term><term>Cytoplasme (métabolisme)</term><term>Espace intracellulaire (MeSH)</term><term>Maladies des plantes (parasitologie)</term><term>Microscopie (méthodes)</term><term>Noyau de la cellule (métabolisme)</term><term>Paroi cellulaire (métabolisme)</term><term>Phytophthora (pathogénicité)</term><term>Phytophthora (physiologie)</term><term>Survie cellulaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Actins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Bryopsida</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Bryopsida</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term><term>Cell Wall</term><term>Cytoplasm</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Actines</term><term>Cytoplasme</term><term>Noyau de la cellule</term><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microscopie</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Bryopsida</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Bryopsida</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Survival</term><term>Intracellular Space</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Espace intracellulaire</term><term>Survie cellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">27027911</PMID><DateCompleted><Year>2018</Year><Month>04</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-2818</ISSN><JournalIssue CitedMedium="Internet"><Volume>263</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>08</Month></PubDate></JournalIssue><Title>Journal of microscopy</Title><ISOAbbreviation>J Microsc</ISOAbbreviation></Journal><ArticleTitle>Interaction between the moss Physcomitrella patens and Phytophthora: a novel pathosystem for live-cell imaging of subcellular defence.</ArticleTitle><Pagination><MedlinePgn>171-80</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/jmi.12395</ELocationID><Abstract><AbstractText>Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.</AbstractText><CopyrightInformation>© 2016 The Authors Journal of Microscopy © 2016 Royal Microscopical Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Overdijk</LastName><ForeName>Elysa J R</ForeName><Initials>EJ</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>DE Keijzer</LastName><ForeName>Jeroen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>DE Groot</LastName><ForeName>Deborah</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schoina</LastName><ForeName>Charikleia</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouwmeester</LastName><ForeName>Klaas</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant-Microbe Interactions, Utrecht University, Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ketelaar</LastName><ForeName>Tijs</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Govers</LastName><ForeName>Francine</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Microsc</MedlineTA><NlmUniqueID>0204522</NlmUniqueID><ISSNLinking>0022-2720</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000199">Actins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000199" MajorTopicYN="N">Actins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019068" MajorTopicYN="N">Bryopsida</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042541" MajorTopicYN="Y">Intracellular Space</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008853" MajorTopicYN="N">Microscopy</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Live-cell imaging</Keyword><Keyword MajorTopicYN="Y">Physcomitrella patens</Keyword><Keyword MajorTopicYN="Y">Phytophthora capsici</Keyword><Keyword MajorTopicYN="Y">Phytophthora infestans</Keyword><Keyword MajorTopicYN="Y">plant-pathogen interaction</Keyword><Keyword MajorTopicYN="Y">subcellular defence</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>02</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>4</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27027911</ArticleId><ArticleId IdType="doi">10.1111/jmi.12395</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre (province)</li><li>Utrecht (province)</li></region><settlement><li>Utrecht</li><li>Wageningue</li></settlement><orgName><li>Université d'Utrecht</li><li>Université de Wageningue</li></orgName></list><tree><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Overdijk, Elysa J R" sort="Overdijk, Elysa J R" uniqKey="Overdijk E" first="Elysa J R" last="Overdijk">Elysa J R. Overdijk</name></region><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><name sortKey="De Groot, Deborah" sort="De Groot, Deborah" uniqKey="De Groot D" first="Deborah" last="De Groot">Deborah De Groot</name><name sortKey="De Keijzer, Jeroen" sort="De Keijzer, Jeroen" uniqKey="De Keijzer J" first="Jeroen" last="De Keijzer">Jeroen De Keijzer</name><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><name sortKey="Ketelaar, Tijs" sort="Ketelaar, Tijs" uniqKey="Ketelaar T" first="Tijs" last="Ketelaar">Tijs Ketelaar</name><name sortKey="Overdijk, Elysa J R" sort="Overdijk, Elysa J R" uniqKey="Overdijk E" first="Elysa J R" last="Overdijk">Elysa J R. Overdijk</name><name sortKey="Schoina, Charikleia" sort="Schoina, Charikleia" uniqKey="Schoina C" first="Charikleia" last="Schoina">Charikleia Schoina</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
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|texte= Interaction between the moss Physcomitrella patens and Phytophthora: a novel pathosystem for live-cell imaging of subcellular defence.
}}
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